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ivis spectrum imaging system  (Revvity)


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    Revvity ivis spectrum imaging system
    Ivis Spectrum Imaging System, supplied by Revvity, used in various techniques. Bioz Stars score: 96/100, based on 37856 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ivis+spectrum+imaging+system/IVIS+optical+imaging+platform/pm42853894-380-6-10
    Average 96 stars, based on 37856 article reviews
    ivis spectrum imaging system - by Bioz Stars, 2026-10
    96/100 stars

    Images

    Related Articles

    Imaging:

    Article Title: Adipose-derived stem cell-derived extracellular vesicles promote critical-sized bone defect repair by enhancing the homing of bone marrow mesenchymal stem cells
    Article Snippet: Then the transfection efficiency was detected by inverted fluorescence microscopy (Leica, Germany). .. Bioluminescence imaging of luciferase-labeled cells in vitro and in vivo To verify the bioluminescence functionality of lentivirally transduced mBMSCs in vitro, the cells were cultured in a 96-well plate, incubated with luciferin substrate, and imaged using an IVIS Spectrum Imaging System (Revvity, USA). ..

    Article Title: CircENO1 drives gastric cancer progression via SPI1-mediated transcriptional activation of ENO1.
    Article Snippet: Background: Circular RNAs (circRNAs) are critical regulators of tumor biology.. However, the role of circENO1 in gastric cancer (GC) remains unclear.. This study investigated the biological function and molecular mechanism of circENO1 in GC.

    Article Title: Dual-glycolipid engineered vaccine platform empowers delivery route customization to intercept tumor lung metastasis.
    Article Snippet: Pulmonary metastasis remains a lethal challenge, necessitating potent mRNA vaccines to establish proactive immune surveillance.. Although lipid nanoparticles (LNPs) are the gold standard Jo ur na l P re -p ro of Journal Pre-proof for delivery mRNA, the "PEG dilemma"—comprising immunogenicity and poor cellular engagement—critically limits their efficacy.. Here, we developed a biocompatibility, PEG-free mRNA platform (SG-LNP) co-modified with sialic acid-dimyristoyl-glycerol (SA-DMG) and monosialotetrahexosylganglioside (GM1), enabling a customizable tissue-targeting delivery pathway.

    Article Title: Combined Gemcitabine and Nab-Paclitaxel Treatment Restores Gut Microbiota Homeostasis in an Orthotopic Pancreatic Ductal Adenocarcinoma Mouse Model.
    Article Snippet: .. For imaging analysis, 150 mg/kg of D-luciferin (Thermo Fisher Scientific, Waltham, MA, USA) was injected intraperitoneally to measure bioluminescence using an IVIS Spectrum imaging system (PerkinElmer, Waltham, MA, USA). .. Living Image software (PerkinElmer) was used for image quantitation and analysis.

    Article Title: Novel EG95-based mRNA vaccines induce long-term protective immunity against echinococcosis.
    Article Snippet: .. Bioluminescence imaging was conducted using an IVIS Spectrum imaging system (PerkinElmer, USA). ..

    Article Title: Sequential KIM-1 targeting and SMOX-responsive release enhance renal delivery of dihydroartemisinin to restore endogenous Klotho and attenuate renal fibrosis.
    Article Snippet: Restoration of endogenous Klotho represents a promising strategy for suppressing renal fibrosis, but efficient delivery of Klotho-restoring therapeutics to injured renal tubules remains challenging.. Here, we developed dihydroartemisinin (DHA)-loaded phosphatidylserine liposomes coated with spermine-modified chitosan (DHAPSLPs@SC) to achieve sequential kidney injury molecule-1 (KIM-1) targeting and spermine oxidase (SMOX)responsive release, thereby enhancing renal delivery of DHA to restore endogenous Klotho and attenuate renal fibrosis.. Dioleoylphosphatidylserine (DOPS) was incorporated to facilitate uptake by KIM-1-expressing injured tubular epithelial cells, whereas the spermine-modified chitosan (SC) coating was designed to promote cargo release in the SMOX-enriched fibrotic cellular environment.

    Article Title: Compound Z247611722 Exhibits Antifungal Activity by Inhibiting Serine Palmitoyltransferase.
    Article Snippet: .. Larval health was monitored daily, and the fungal load was assessed daily with BLI using an IVIS Spectrum imaging system (Revvity). .. 10 μL of 40 μg/g D-Luciferin was injected into the hemocoel prior to every imaging session, and the larvae were transferred to a black 12-well plate with a transparent bottom (IBL Baustoff + Labor GmbH, Austria) at 37°C for 10min73,75,76.

    Article Title: LAMTOR5-AS1 predicts poor prognosis and regulates glioma progression via miR-4653-5p/TCF7 axis
    Article Snippet: For in vitro transduction, U87MG cells were cultured in 173 6-well plates until reaching 60-70% confluence, they were transduced with 10 μl of CON101 174 lentiviral vector (3.5 × 108 TU/ml, Ubi-MCS-firefly-Luciferase- IRES-Puromycin; GeneChem, 175 Shanghai, China) and 40 μ l of 25× HiTransG Polution Enhancement Reagent (GeneChem) for 8 h 176 at 37 °C with 5% CO2 . .. Transduction efficiency was verified 72 h post- infection by quantifying 177 bioluminescence signals using the IVIS Spectrum imaging system (PerkinElmer, Waltham, MA, 178 USA). .. 179 For in vivo imaging, 6-week-old male BALB/c nude mice (Vital River Laboratories) were 180 stereotactically implanted with 5 × 105 luciferase-expressing U87MG cells.

    Luciferase:

    Article Title: Adipose-derived stem cell-derived extracellular vesicles promote critical-sized bone defect repair by enhancing the homing of bone marrow mesenchymal stem cells
    Article Snippet: Then the transfection efficiency was detected by inverted fluorescence microscopy (Leica, Germany). .. Bioluminescence imaging of luciferase-labeled cells in vitro and in vivo To verify the bioluminescence functionality of lentivirally transduced mBMSCs in vitro, the cells were cultured in a 96-well plate, incubated with luciferin substrate, and imaged using an IVIS Spectrum Imaging System (Revvity, USA). ..

    In Vitro:

    Article Title: Adipose-derived stem cell-derived extracellular vesicles promote critical-sized bone defect repair by enhancing the homing of bone marrow mesenchymal stem cells
    Article Snippet: Then the transfection efficiency was detected by inverted fluorescence microscopy (Leica, Germany). .. Bioluminescence imaging of luciferase-labeled cells in vitro and in vivo To verify the bioluminescence functionality of lentivirally transduced mBMSCs in vitro, the cells were cultured in a 96-well plate, incubated with luciferin substrate, and imaged using an IVIS Spectrum Imaging System (Revvity, USA). ..

    In Vivo:

    Article Title: Adipose-derived stem cell-derived extracellular vesicles promote critical-sized bone defect repair by enhancing the homing of bone marrow mesenchymal stem cells
    Article Snippet: Then the transfection efficiency was detected by inverted fluorescence microscopy (Leica, Germany). .. Bioluminescence imaging of luciferase-labeled cells in vitro and in vivo To verify the bioluminescence functionality of lentivirally transduced mBMSCs in vitro, the cells were cultured in a 96-well plate, incubated with luciferin substrate, and imaged using an IVIS Spectrum Imaging System (Revvity, USA). ..

    Cell Culture:

    Article Title: Adipose-derived stem cell-derived extracellular vesicles promote critical-sized bone defect repair by enhancing the homing of bone marrow mesenchymal stem cells
    Article Snippet: Then the transfection efficiency was detected by inverted fluorescence microscopy (Leica, Germany). .. Bioluminescence imaging of luciferase-labeled cells in vitro and in vivo To verify the bioluminescence functionality of lentivirally transduced mBMSCs in vitro, the cells were cultured in a 96-well plate, incubated with luciferin substrate, and imaged using an IVIS Spectrum Imaging System (Revvity, USA). ..

    Incubation:

    Article Title: Adipose-derived stem cell-derived extracellular vesicles promote critical-sized bone defect repair by enhancing the homing of bone marrow mesenchymal stem cells
    Article Snippet: Then the transfection efficiency was detected by inverted fluorescence microscopy (Leica, Germany). .. Bioluminescence imaging of luciferase-labeled cells in vitro and in vivo To verify the bioluminescence functionality of lentivirally transduced mBMSCs in vitro, the cells were cultured in a 96-well plate, incubated with luciferin substrate, and imaged using an IVIS Spectrum Imaging System (Revvity, USA). ..

    Software:

    Article Title: CircENO1 drives gastric cancer progression via SPI1-mediated transcriptional activation of ENO1.
    Article Snippet: Background: Circular RNAs (circRNAs) are critical regulators of tumor biology.. However, the role of circENO1 in gastric cancer (GC) remains unclear.. This study investigated the biological function and molecular mechanism of circENO1 in GC.

    Fluorescence:

    Article Title: Dual-glycolipid engineered vaccine platform empowers delivery route customization to intercept tumor lung metastasis.
    Article Snippet: Pulmonary metastasis remains a lethal challenge, necessitating potent mRNA vaccines to establish proactive immune surveillance.. Although lipid nanoparticles (LNPs) are the gold standard Jo ur na l P re -p ro of Journal Pre-proof for delivery mRNA, the "PEG dilemma"—comprising immunogenicity and poor cellular engagement—critically limits their efficacy.. Here, we developed a biocompatibility, PEG-free mRNA platform (SG-LNP) co-modified with sialic acid-dimyristoyl-glycerol (SA-DMG) and monosialotetrahexosylganglioside (GM1), enabling a customizable tissue-targeting delivery pathway.

    Analysis:

    Article Title: Combined Gemcitabine and Nab-Paclitaxel Treatment Restores Gut Microbiota Homeostasis in an Orthotopic Pancreatic Ductal Adenocarcinoma Mouse Model.
    Article Snippet: .. For imaging analysis, 150 mg/kg of D-luciferin (Thermo Fisher Scientific, Waltham, MA, USA) was injected intraperitoneally to measure bioluminescence using an IVIS Spectrum imaging system (PerkinElmer, Waltham, MA, USA). .. Living Image software (PerkinElmer) was used for image quantitation and analysis.

    Injection:

    Article Title: Combined Gemcitabine and Nab-Paclitaxel Treatment Restores Gut Microbiota Homeostasis in an Orthotopic Pancreatic Ductal Adenocarcinoma Mouse Model.
    Article Snippet: .. For imaging analysis, 150 mg/kg of D-luciferin (Thermo Fisher Scientific, Waltham, MA, USA) was injected intraperitoneally to measure bioluminescence using an IVIS Spectrum imaging system (PerkinElmer, Waltham, MA, USA). .. Living Image software (PerkinElmer) was used for image quantitation and analysis.

    Tissue:

    Article Title: Sequential KIM-1 targeting and SMOX-responsive release enhance renal delivery of dihydroartemisinin to restore endogenous Klotho and attenuate renal fibrosis.
    Article Snippet: Restoration of endogenous Klotho represents a promising strategy for suppressing renal fibrosis, but efficient delivery of Klotho-restoring therapeutics to injured renal tubules remains challenging.. Here, we developed dihydroartemisinin (DHA)-loaded phosphatidylserine liposomes coated with spermine-modified chitosan (DHAPSLPs@SC) to achieve sequential kidney injury molecule-1 (KIM-1) targeting and spermine oxidase (SMOX)responsive release, thereby enhancing renal delivery of DHA to restore endogenous Klotho and attenuate renal fibrosis.. Dioleoylphosphatidylserine (DOPS) was incorporated to facilitate uptake by KIM-1-expressing injured tubular epithelial cells, whereas the spermine-modified chitosan (SC) coating was designed to promote cargo release in the SMOX-enriched fibrotic cellular environment.

    Transduction:

    Article Title: LAMTOR5-AS1 predicts poor prognosis and regulates glioma progression via miR-4653-5p/TCF7 axis
    Article Snippet: For in vitro transduction, U87MG cells were cultured in 173 6-well plates until reaching 60-70% confluence, they were transduced with 10 μl of CON101 174 lentiviral vector (3.5 × 108 TU/ml, Ubi-MCS-firefly-Luciferase- IRES-Puromycin; GeneChem, 175 Shanghai, China) and 40 μ l of 25× HiTransG Polution Enhancement Reagent (GeneChem) for 8 h 176 at 37 °C with 5% CO2 . .. Transduction efficiency was verified 72 h post- infection by quantifying 177 bioluminescence signals using the IVIS Spectrum imaging system (PerkinElmer, Waltham, MA, 178 USA). .. 179 For in vivo imaging, 6-week-old male BALB/c nude mice (Vital River Laboratories) were 180 stereotactically implanted with 5 × 105 luciferase-expressing U87MG cells.

    Infection:

    Article Title: LAMTOR5-AS1 predicts poor prognosis and regulates glioma progression via miR-4653-5p/TCF7 axis
    Article Snippet: For in vitro transduction, U87MG cells were cultured in 173 6-well plates until reaching 60-70% confluence, they were transduced with 10 μl of CON101 174 lentiviral vector (3.5 × 108 TU/ml, Ubi-MCS-firefly-Luciferase- IRES-Puromycin; GeneChem, 175 Shanghai, China) and 40 μ l of 25× HiTransG Polution Enhancement Reagent (GeneChem) for 8 h 176 at 37 °C with 5% CO2 . .. Transduction efficiency was verified 72 h post- infection by quantifying 177 bioluminescence signals using the IVIS Spectrum imaging system (PerkinElmer, Waltham, MA, 178 USA). .. 179 For in vivo imaging, 6-week-old male BALB/c nude mice (Vital River Laboratories) were 180 stereotactically implanted with 5 × 105 luciferase-expressing U87MG cells.



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    <t>In</t> <t>vivo</t> and ex vivo validation of TNBC targeting by Dextran‐AF680. (A) The average radiant efficiency of Dextran‐AF680 peaked at 3 h after intravenous injection, with significant differences between 1 h and 3 h and between 3 h and 6 h ( p = 0.0313, n = 6) by in vivo fluorescence imaging. (B–D) The biodistribution of Dextran‐AF680 in orthotopic models was monitored using conventional fluorescence imaging at 3, 8, and 24 h. At each time point, mice were euthanized, and tumors and organs were harvested for ex vivo fluorescence imaging. Ex vivo organ imaging revealed an increasing trend in Dextran‐AF680 uptake by TNBC tumors, the liver, and the kidneys ( p = 0.0022, p = 0.0043, n = 6). (E, F) Tumors were cryo‐sectioned, stained, and imaged on a confocal microscope (blue, Hoechst; red, Dextran‐AF680; green, F4/80‐AF488; scale bars, 25 μm) Strong, well‐demarcated fluorescent signal was visible ex vivo, showing a high degree of overlap between the F4/80 targeted pan macrophages and Dextran‐targeted cells. Furthermore, quantification of fluorescent signals confirmed a clear signal from intravenously injected Dextran, further supporting its utility as an imaging probe for in vivo targeting of M2 macrophages. (G, H) Dextran‐targeted cells (red) did not overlap with CD86‐positive M1‐like macrophages (green). Data are presented as the mean ± standard deviation (SD).
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    Image Search Results


    In vivo and ex vivo validation of TNBC targeting by Dextran‐AF680. (A) The average radiant efficiency of Dextran‐AF680 peaked at 3 h after intravenous injection, with significant differences between 1 h and 3 h and between 3 h and 6 h ( p = 0.0313, n = 6) by in vivo fluorescence imaging. (B–D) The biodistribution of Dextran‐AF680 in orthotopic models was monitored using conventional fluorescence imaging at 3, 8, and 24 h. At each time point, mice were euthanized, and tumors and organs were harvested for ex vivo fluorescence imaging. Ex vivo organ imaging revealed an increasing trend in Dextran‐AF680 uptake by TNBC tumors, the liver, and the kidneys ( p = 0.0022, p = 0.0043, n = 6). (E, F) Tumors were cryo‐sectioned, stained, and imaged on a confocal microscope (blue, Hoechst; red, Dextran‐AF680; green, F4/80‐AF488; scale bars, 25 μm) Strong, well‐demarcated fluorescent signal was visible ex vivo, showing a high degree of overlap between the F4/80 targeted pan macrophages and Dextran‐targeted cells. Furthermore, quantification of fluorescent signals confirmed a clear signal from intravenously injected Dextran, further supporting its utility as an imaging probe for in vivo targeting of M2 macrophages. (G, H) Dextran‐targeted cells (red) did not overlap with CD86‐positive M1‐like macrophages (green). Data are presented as the mean ± standard deviation (SD).

    Journal: Cancer Medicine

    Article Title: In Vivo Immunoscoring Technique to Monitor Anti‐ CD47 Immunotherapy Efficacy in Triple‐Negative Breast Cancer

    doi: 10.1002/cam4.72232

    Figure Lengend Snippet: In vivo and ex vivo validation of TNBC targeting by Dextran‐AF680. (A) The average radiant efficiency of Dextran‐AF680 peaked at 3 h after intravenous injection, with significant differences between 1 h and 3 h and between 3 h and 6 h ( p = 0.0313, n = 6) by in vivo fluorescence imaging. (B–D) The biodistribution of Dextran‐AF680 in orthotopic models was monitored using conventional fluorescence imaging at 3, 8, and 24 h. At each time point, mice were euthanized, and tumors and organs were harvested for ex vivo fluorescence imaging. Ex vivo organ imaging revealed an increasing trend in Dextran‐AF680 uptake by TNBC tumors, the liver, and the kidneys ( p = 0.0022, p = 0.0043, n = 6). (E, F) Tumors were cryo‐sectioned, stained, and imaged on a confocal microscope (blue, Hoechst; red, Dextran‐AF680; green, F4/80‐AF488; scale bars, 25 μm) Strong, well‐demarcated fluorescent signal was visible ex vivo, showing a high degree of overlap between the F4/80 targeted pan macrophages and Dextran‐targeted cells. Furthermore, quantification of fluorescent signals confirmed a clear signal from intravenously injected Dextran, further supporting its utility as an imaging probe for in vivo targeting of M2 macrophages. (G, H) Dextran‐targeted cells (red) did not overlap with CD86‐positive M1‐like macrophages (green). Data are presented as the mean ± standard deviation (SD).

    Article Snippet: Bioluminescence signals were acquired 10 min post‐injection using an IVIS Spectrum CT system (PerkinElmer, USA).

    Techniques: In Vivo, Ex Vivo, Biomarker Discovery, Injection, Fluorescence, Imaging, Staining, Confocal, Microscopy, Standard Deviation